PPT Slide
THE REPLICATION OF DNA IN ESCHERICHIA COLI
1) What were M&S attempting to measure in these experiments?
2) What techniques did they employ? How do these techniques work?
a) what is the importance of density-gradient centrifugation?
b) why is the measurement of cell density so important to M&S? See figure 3
3) What does Figure 2 tell us? How was it obtained?
4) What does Figure 4 tell us? What are the key photos here?
5) What is the interpretation of Figure 9?
a) Why does density of DNA increase upon heat denaturation?
b) What is the importance of fig. 9, B? Why is fig. 9, C an important control?