PPT Slide
Gene Mutant phenotype Gene Product Function
uvrA sensitivity to UV ATPase subunit of exinuclease damage recognition, uvrB
uvrB “ B subunit of exinuclease binding target of uvrC,
uvrC “ C subunit of exinuclease 5’ endonuclease
uvrD “ Helicase II removes excised oligomer
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recA deficiency in recombination RecA DNA strand exchange,
and repair protease - initiates SOS
recB deficient in recombination, exonuclease V* (ATPase) ATPase
recC “ exonuclease V subunit DNA binding
recD “ exonuclease V subunit (58K) ?
sbcB def icient in mismatch repair exonuclease I exonuclease
dam UV sensitive, mutator methylase (GATC) tags “old” strand
mutS “ MutS binds to mismatched bp
mutL “ MutL w/MutS, activates MutH
mutH “ endonuclease cleaves at hemimethylated
lexA SOS constitutive repressor repressor for many genes
ada sensytivity to alkylation G methyl transferase de-methylates G
Some genes involved in DNA repir in E. coli