The model was generated by creating a new pdb file with Hyperchem molecular modeling software. The pdb is a synthesis of 1dul (Batey, et al., 2000) and 2ffh (Keenan, et al., 1998). The M domain of 2ffh was isolated and superimposed on the M domain fragments of 1dul. The 1dul protein atoms were then removed. A model alpha helix representing the prelysozyme signal sequence (ATOMNO 1 - 134) was then inserted into the hydrophobic groove of the M domain. Free energy was reduced with 1000 cycles of a steepest descent molecular mechanics algorithm (Hyperchem).