PPT Slide

2) electrophoresis

+

-

DNA

migration to

+ end of gel

agarose gel

DNA digested with a restriction enzyme

1) digested DNA loaded in gel

3) DNA fragments

blotted onto filter

4) filter hybridized

with labeled gene

fragment

5) bound label

detected via

autoradiography

or luminescence

+

-

Southern Blotting

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