Replication genes/proteins of E. coli
Gene Phenotype Protein/function
dnaA slow-stop - defective origin initiation dnaA/initiation at ori
dnaB quick-stop dnaB/Helicase
rep slowed fork movement helicase
dnaE quick-stop alpha subunit- polIII
dnaN loss of processivity beta subunit of polIII
dnaQ mutator eplsilon subunit of polIII
dnaX quick-stop gamma and tau subunits
polA defective in DNA repair polI
polB defective in DNA repair polII
ori non-viable origin of replication
gyrA quick and slow-stop DNA gyrase alpha subunit;nicking,closing
gyrB quick and slow-stop gyrase subunit beta; ATPase
ssb quick-stop;defective in repair& SSB (single-strand binding protein)
dnaT slow-stop; stable replication primosome assembly
priA,B,C slow-stop;stable replication primosome subunits
dnaC slow or quick-stop dnaC;complex with dnaB-loads helicase/primase